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Introduction
to Effective Microorganisms (EM)
EM
Bokashi; EM and Antioxidant Activity
Notice and Disclaimer
(this notice
appears
on each page on this site)
A few of the
uses for
EM culture and products which are discussed herein may
be contrary
to regulatory rules or guidelines in your country, state, province,
county
or region. Further, some practices may be frowned upon by
qualified
health professionals, and some practices could be dangerous to human
health,
or could be dangerous to animal health (or crop health!) if
performed
or processed incorrectly. This site and these pages are
offered for
educational and informational purposes only. If you choose to
use
EM or any other microbial inoculant products in any way for any
application,
you must first check with your local and national authorities to
determine
if your planned use complies with all applicable rules, regulations and
requirements. If you choose to use EM for any purposes
involving
human ingestion (or placement upon skin, etc.), I recommend that
you first research all relevant information available in the
literature and
on the web carefully, and review the recommendations in the
regulatory guidelines for
your
country or region. Further, if brewing EM products
for
human or animal consumption, you will also wish to employ common sense
and careful techniques.
Any
statements and opinions
offered in these pages are my opinions only offered in reportorial and
informational mode, and do not reflect in any way the views of any
creators,
producers, distributors, marketer or vendors of various EM-type
cultures
or products. Please note that I (the author) am not in any way
associated
with any of the above-referenced persons or organizations, although I
may
occasionally perform consulting for some companies, entities and
individuals
in the EM field. All opinions and statements remain my own
reportage
and opinions, and at times my opinions and/or practices may differ
wildly
from those of the various creators, producers, distributors
or vendors
of EM products or EM-like products.
Making
EM Bokashi
Bokashi, if you recall, is
a composted material produced by EM culturing of bran (or other
substances,
including agricultural or other wastes.) The name is often
used as
well for the compost produced by fermenting kitchen wastes in a bucket;
the only difference here is that the quality of the stuff put in the
bucket
may not have been as high nor clean as a bokashi made from food-grade
bran
and other food-grade materials, and the intended use for the bokashi
batches
may be different.
Making Bokashi for
Animal Feed
These instructions and
hints are geared toward making a pure, clean, "sanitary" bokashi from
bran
(or bran and granulated kelp), rather than a more hit-or-miss (and
"dirty")
kitchen waste composter, which pretty much takes care of itself, so
long
as it is regularly inoculated with EM.
To make EM Bokashi for use
in mixing (3% to 5%) with livestock or poultry feed, or for sprinkling
on animal wastes and on the ground in animal pens, or for seeding
compost
or solid, take Activated EM (aka EM Extension), mix 1:1:100
with molasses and water, and add slowly to wheat bran (or rice bran),
with
perhaps some granulated kelp, a bit of sea salt, and a bit of Azomite
rock
dust as well (all optional), and stir THOROUGHLY until the material is
about 30% moist. The EM folks say that it should be moist
enough
to form a clump which sticks together if you squeeze some in your hand,
but should be dry enough to crumble again when touched
lightly. I
find that this works best myself. This moistened mixture of
bran
(or bran and kelp, etc.) is then placed in an airtight container to
ferment
anaerobically for 5 to 14 days, depending upon temperatures (cooler
takes
longer...). How to make an airtight container? Read
the section
below...., but first, some practical tips on making bokashi:
Tips and Hints
These hints are geared,
as are the paragraphs above, toward making a pure, clean "sanitary"
bokashi
from bran (or bran and kelp), rather than a more hit-or-miss kitchen
waste
composter, which pretty much takes care of itself, so long as it is
regularly
inoculated.
- I usually mix bokashi in a 2 gallon, 3 gallon,
5 gallon or
6 gallon round plastic bucket
- I recommend using a bucket of at least 2
gallons in size, to allow adequate
room for materials
- IMPORTANT! Fill the container only
half-full of dry bran (and any
other additives, such as kelp granules, salt, rock dust!)
- if you have added kelp, sea salt, or rock dust
to the dry bran, it is very
important to mix that in well with the bran, while still dry, before
adding
the liquid slowly.
- You must only half-fill the bucket, because the
material may expand up
to 20% as you add the water/EM/molasses mix, and you need "head room"
so
that you can easily stir the material without spilling it all over the
floor!
- if you live in a temperate zone, and need hints
on how to keep your batches
of bokashi warm in order to accelerate brewing, please see the
sub-section
within the Making Activated EM (aka EM Extension)
section
entitled "Keeping Batches of AEM or Bokashi Warm in a Cold
Climate"
Making an Airtight Container
Most folks put the moistened and inoculated bran mixture in a clean
plastic pail, and fill the pail to perhaps 1/3 to 2/3 full.
Then,
place a clean plastic bag (perhaps a gallon plastic food storage bag,
over
the entire surface of the bran mix, and then place a round dinner plate
or plastic lid from another type of bucket – chosen to be
just smaller
than the diameter of the inside of your bucket at the top of the bran
mix
– over the plastic bag to form a seal. Then, place
a stone or small
brick on the plate or other circular disk to press it down and keep air
out. Do not open this air seal till the mix is done, except
perhaps
to check (to peek) occasionally.
Checking to See if Done
As mentioned above, bokashi may take anywhere from 3 days (about 97
degrees F) to 5 or 6 days (about 91 degrees F), to over 10 to 14 days
(72
to 80 degree F range.) When the Bokashi is done, it should have a
wonderful,
clean, sweet and sour smell. If you smell a putrid odor, then
the
moisture level was too high and butyric acid was developed during
fermentation,
and the batch should be discarded, the bucket cleaned, and the process
started again. Likewise, any fuzzy molds or smelly molds indicate that
something went wrong. The EM folks stress that it is indeed perfectly
alright
to see some fungal mycelium (fine threadlike fungus, white to
off-white)
in the finished bokashi, but you should not see fuzzy molds.
I have
been lucky; I have made twelve batches (each from 1 gallon to 4 gallons
in volume) of bokashi (some for human use, some for animal feed, some
for
treating animal wastes) in my first few months of using EM organisms,
and
each has turned out very well.
go
back
to top of page and table of contents
Storing
and Using EM Bokashi
Storage
Once your bokashi is finished,
store it in a cool, dark place. If stored totally or
nearly anaerobically,
under the same conditions (anaerobic) as it was made, it will
keep
many months, and will actually improve with time. EM Bokashi,
when
stored in its moist state, but not anaerobically (in other words, not
sealed) will keep for about 2 weeks at 78 degrees or above, about 3
weeks
or longer at 68 to 70 degrees, and much longer at cooler
temperatures.
If dried and then stored dried, it will keep for at least 2 months at
78 degrees or above, and longer at cooler temperatures.
Uses
I suggest mixing a bit of
EM bokashi with feed for your livestock or poultry each day; the
official
EM channels recommend 4% to 5% of EM by volume be added to
feed.
Be careful if you have poultry; if the bokashi is used in the damp
form,
it could conceivably encourage the nearby grains to get moldy in warm
weather,
and most birds (chickens, ducks, geese, turkeys, etc.) do not tolerate
the presence of "bad" molds in their feed well; this can be
fatal.
A lot of EM organizations recommend, therefore, that you use only dried
bokashi for supplementing poultry feed, and that you do not use damp
bokashi
for this purpose when feeding birds. Despite the warning from
the
EM folks, I personally do not have the time or patience to dry bokashi
for poultry feed, and therefore I do add damp bokashi to my poultry
feed
(mixed cracked grains, often called "scratch") on a daily
basis.
So far, I have had no problem -- all the feed is consumed within the
next
12 hours, and there is little left.
If you are even considering
eating a tiny amount of bran or bran/kelp powder bokashi as a (human)
nutritional
supplement, please do so at your own risk and be sure that the batch is
relatively young and fresh, and that the stuff smells clean, sweet and
sour, and please start with only very tiny amounts!
Bokashi may also be crumbled
and dispersed over animal wastes, soil in gardens or in animal pens, or
on compost, to drastically change the micro-flora.
go
back
to top of page and table of contents
Bottle Labels, Labeling,
Uses and Local
Regulations
and Laws (a big one!)
and
National,
State/Province/County Regulations
and
Regulatory Compliance
Brief Introduction
In case you have not noticed, our modern world has become quite
complex,
with literally thousands of regulatory agencies and laws at the
national,
state, provincial or regional level, and even at the city or town
level.
Not so surprisingly, in many countries and regions, these regulations
may
specify exactly what may or may not be:
- ingested by humans
- fed to livestock or pets
- used in production of food-producing animals
- applied to soils
- applied to wastes (e.g., agricultural, yard,
household or animal wastes)
- sprayed in the air
- applied to septic tanks or other bulk liquid
waste processing tanks
Also, it is likely not surprising that products containing cultures of
microorganisms are often subject to regulatory requirements and
restrictions.
I will tell you far more about this below! Read on!
Culture Uniformity Across All
Labels
As already stated in two earlier sections, from a microbiological point
of view, all the cultures in each and every EM1 (aka EM-1,
etc.) label (no, we are not talking about EM2, EM3 and EM4,
which
are still sold
in some countries) is identical, and regardless of what certain stores
may try to tell you. Read this closely:
- Identical cultures across all bottle
labels!
- Identical cultures across all bottle
labels!
- ...sorry for getting so strident,
but this addresses a recurring myth!
However, solely due to regulatory and regulatory compliance reasons and
marketing reasons, in many countries (especially, lately, the USA...)
and
regions (states, provinces, counties, federal law, etc.), there are
several
labels available (e.g., Waste, Septic Waste, Soil, Agricultural, etc.)
in order to address these requirements and restrictions. Read
on!
An Example of Regulations,
Claims Uses, and Labeling
An example follows for the USA only, but is applicable almost anywhere:
due to the many federal (usually EPA or FDA, sometimes USDA) laws and
regulations
and certification requirements, EMRO USA has recently (late 2002) been
forced to change the labels on their EM·1
bottles.
By the way, the new prefix for EM1 is now really
EM·1, where the floating dot, unfortunately, while
it can be reproduced on
web pages and in MS Word and some other word processors, cannot be
reproduced
in text (non-HTML) e-mail messages and even in some HTML e-mail as
well.
So, if you ever notice that someone appears to have typed EM?1, that
means
they typed EM·1, but that their
email client (or
yours) represents the dot as a question mark.
Getting back to the EM bottles sold in the USA,
for now, due to regulatory
compliance issues, EMRO USA will be selling only three labels, as of
late
2002:
- EM·1 Soil Amendment
(approval currently secured for use in 13 states; may actually be legal
in far more... the approvals are still in process)
- EM·1 Waste
Treatment: For Septic Systems (salable in 45
states, and may be
legal in more; some approvals are still in process)
- EM·1 Waste
Treatment (salable in 48 states)
Believe it or not, even with this very restrictive labeling (you will
notice
that no labels read "Livestock" or "Agricultural" or "Animal"), it is
technically
illegal for an EM store or an EM CEMP to sell certain labels to folks
who
live in certain states. So, for now, as the new labels
appear, the
safest thing (if you wanna be sure you don't get the state pissed
off...)
to do is to purchase only the EM Waste Treatment
label, since
that label is legal to be sold in at least 48 states (plus Canada,
Mexico
and the Caribbean). Incidentally, the Waste
Treatment: For
Septic Systems product is a close runner-up, as it
is currently
legal in 46 states, and apparently in Mexico (Caribbean and Canadian
information
not available yet), while the Soil product is now legal in only about
15
states. Conversely, regulatory requirements or certification
and
registration requirements currently limit sale of the other two labels
to a much smaller list of states. The list of states in which each
product
may be sold, by the way, is available on the EMRO USA website.
Of course, as time passes, and as EMRO USA goes
thru more certification
and compliance steps (often expensive licenses...) more and more states
will appear on the "okay to buy" lists for each label. There
is a
good chance that there may even eventually be a label reading "Human
Use".
So, bottom line: Unless I am feeling that I must
fanatically comply
with state regulations on what label I should really be using, I buy
any
EM label the vendor will sell me -- it does not matter at all -- as
long
as the stuff has not passed expiration date, and I use the same bottle
for everything: animal feed, animal water, animal waste, household,
human
use, ingestion, making weird monatomic mineral supplements, etc.
An Example Set in the USA --
Animal Feed
Under FDA rules, it is technically illegal to administer EM and EM
products to any animal in the USA, since its use in animal feed is not
yet approved; this is simply because of the fact that in a mechanized
farming
operation, animals would not have the ability to choose freely whether
to ingest EM. There is a good chance that EMRO will
eventually
be able to comply with various certification requirements of the FDA,
and
therefore gain approval for the use of EM in animal feed.
The reality is the FDA (or USDA) is extremely
unlikely to come after
someone who chooses to fed this stuff to their dog, cat or livestock or
other animals. The reality is that in the USA we live in a
fairly
free society, and people, once they have purchased a product, are
largely
free to do whatever they wish with it, regardless of what the label
said
or did not say. However, a vendor or marketer is in a very
different
position: the FDA would come down very hard on them if they did any of
the following:
- recommended a technically-illegal practice
- intimated that such a use of their product
would be helpful
- marketed their product as suitable for that
illegal use
- labeled their product as suitable for that
illegal use.
How Do Other Countries View Use of EM in
Animal Feed?
The vast majority of countries in the world do not regulate what may
or may not be fed to animals. While these liberal guidelines
do allow
free use of EM in these countries, this free-market approach is
actually
worrisome, since it could allow all kinds of toxic things into
the
diet of animals, and also into the human food chain. Of the few
countries
which do regulate such things, we have already dealt with the current
situation in the USA above, and, of the remaining countries, EM is
registered
(and allowed) for animal consumption in Austria, Sweden, Denmark, and
China.
It appears that some other countries may actually have regulations on
the
books which might forbid use of many microbial products (including EM)
in animal feeds, but if so, they appear to tolerate and allow the use
of
EM1 because there are no other solutions that are as effective in
eliminating
odor and environmental problems, and at a price that farmers can easily
afford.
So, the Bottom Line Is....
The actual reality of EM use in the USA (continuing the example above,
since the USA is one of the few countries where its use in animal feed
is not yet approved) is that MANY folks use it, with great results, in
animal feed and water, and continue to do so, just as do many farmers
across
the world. Many private EM consultants in the USA continue to
recommend
EM for these purposes, as do many organic farming and beyond
organic (or uber
organic) farming consultants (but notice, they are not
vendors of
EM, but rather independent!.....) That does not
stop thousands
of small American farmers and pet-owners from feeding it to their
animals,
and frankly, the FDA does not care about that.
Likewise, many humans in the USA and across the
world continue to ingest
EM every day, and the FDA could care less about that. Since
FDA rules
are different for humans and animals, it is actually legal for a
vendor to market EM for human consumption, so long as all ingredients
used
in the fermented product were food-grade and recognized as
safe.
EM
and Antioxidant Activity, also EM-X
One point which Dr. Higa
and other advocates of EM have repeatedly asserted is that the EM
organisms
(in anaerobic fermentation) produce a plethora of antioxidant
substances
which are beneficial to humans and animals (and to soil, ponds,
streams,
etc.). Dr. Higa goes further, and asserts that while
"undesirable"
decay bacteria are primarily oxidative in their activities, the
organisms
in EM are primarily reducing (antioxidant) in their activities, and
thus
help to preserve and amplify healthful and coherent life-enhancing
energies
and patterns in water and other substances. Indeed, it has been noted
many
times by Dr. Higa and the EMRO organization that even Activated EM
(aka EM Extension) liquid is loaded with antioxidants, and it must be
noted
that some folks in Japan and the USA (and elsewhere!) brew Activated EM
to ingest as an antioxidant and probiotic health drink. It
has also
been asserted and noted by the EMRO organization and EM users, as well
as this researcher, that even when Activated EM (AEM) has passed 30 to
60 days in age and potentially lost some of the viability of the
culture,
it still remains a potent antioxidant liquid with a shelf life -- so
long
as it is kept airtight and in relatively anaerobic conditions -- of
many
years. Indeed, research in my laboratory and elsewhere -- and
this
has been confirmed by many folks who have worked with EM, including
students
of Dr. Higa -- shows that for as long as fermentation continues, the
antioxidant
(aka reducing) potency of the EM-1 or AEM steadily increases, and, of
course,
concomitantly, the pH will continue to decrease, however slowly.
A Brief list of Antioxidants and
Related Nutrients Produced by
EM1
Some of the antioxidants and related nutrients which have been found
in EM1 and EM1 products such as EM-X by researchers, as quoted by Dr.
Higa,
EMRO Japan and others, have been :
- Inositol
- Ubiquinone
- Quinones
- Saponins
- low molecular-weight polysaccharides (these
have been documented to have
a wide range of interesting and healthful properties)
- polyphenols
- chelated minerals, including trace minerals
From Which Organisms Do
the Above Antioxidants and Nutrients Come?
Dr. Higa has repeatedly
reminded researchers and EMRO staffers that it is difficult to try to
lay
the responsibility for the production of the antioxidants at the feet
of
any one of the species or organism in EM1, and rather, that it is far
more
accurate to say that the antioxidants are produced by a complex,
interdependent
and synergistic relationship between the three major groups of
organisms,
as well as the helper organisms. However, the current author is
convinced
that many of the antioxidants are produced by the phototrophic bacteria
under anaerobic fermentation, but with the assistance of the other
organisms
as well.
A More Detailed List of
Antioxidants and Nutrients Likely to be
Found in EM1 and Related Products
Recently I had reported on my raw foods e-mail list group some of the
healthful experiences (increases in robustness, health, stamina and
vitality)
which I have found in ingesting human brew versions of Activated EM and
especially, my most recent batch of homemade EM-X-like brew, which I
have
called GoldenBran Kelp brew. While not an overly-analytic person, I
eventually
did some further research on the antioxidants and other nutrients
present
in EM, and I decided to go well beyond the assertions made by Dr. Higa
about the presence of certain healthful substances, and instead I
performed
a massive survey of the peer-reviewed scientific literature for the
substances
produced by the organisms in EM when in anaerobic fermentative
mode.
The results of my search exceeded my wildest imaginings... I now
realize
why I have recently -- since starting to consume AEM and BKMC -- cut
way
back on my intake of nutritional antioxidant supplements (as guided by
my body sense and intuition). Here, below, are some of my findings;
please
note that nutrient brews made with good-quality wheat bran or rice bran
will be even higher in many of these agents than the impressively
strong
levels found in AEM and similar brews.
The three phototrophic organisms alone in the AEM
produce a whole slew
of incredibly powerful antioxidants, and it appears that the yeast and
lactic acid bacteria produce a few more. Some of the nutrient compounds
created by the phototrophic organisms under a wide variety of growth
conditions
include immune-enhancing complexes, and powerful nutrients, some of
which
(ALA, COQ10, chlorophylls, beta-glucans, carotenoids, indoles [remember
the anti-cancer stuff in broccoli?], octacosanol, DMSO, butryrates (the
potent antioxidant found in raw butter), lycopene, vitamin B-12,
canthaxanthin,
zeaxanthin, glutathione, etc.) would cost a small fortune in a
nutritional
supplement store; some of the compounds are alleged also to have
anti-fatigue
and anti-diabetic properties as well. A more complete
analysis follows:
The three phototrophic reducing organisms in the
EM formula for the
USA are the following Rhodobacter (formerly Rhodopseudomonas; synonyms:
"Rhodobacillus" and "Rhodomonas") species:
- R. spheriodes
- R. capsulata
- R. palustris
Dr. Higa claims that they are the secret heart of his brew, and that
they
force the antioxidative (aka reducing) environment for the other
organisms,
and then he wanders into pseudo-scientific pop language and claims that
the phototrophic organisms are "nuclear-powered" (he may be attempting
here to describe the activities of the chromatophores in R. spp. ...),
and that they emit "high frequency gravity waves...". As you may guess,
that kinda stuff loses me, but the verifiable facts from the
peer-reviewed
scientific literature are even more impressive!
All three purple non-sulphur bacteria are capable
of producing
hydrogen
in anaerobic conditions, all seem to contribute to the production of
the
primeval hydrogen ion (hydride) antioxidant species during fermentation
(as is found in MegaHydrin and also in alkaline electrolyzed reduced
water,
or ERW), and all seem to contain and to make a wide range of more
complex
biochemical antioxidants and related nutrient substances, including
(and
this is straight from the peer-reviewed scientific literature.....):
vitamin
C, lots of B vitamins, antivirals, bacteriochlorophyll, carotenoids
(incl.
lycopenes, zeaxanthin and a novel purple carotenoid called
rhodobacterioxanthin),
cobalamin (aka vitamin B12), retinols (related to vitamin A),
peroxidase
species, oxidoreductase species, reductase enzyme species, methionine
side
chain species, quinones, ubiquinone (aka coenzyme Q10),
flavonoids,
superoxide dismutase species, catalase-peroxidase species (powerful
antioxidants),
dimethylsulfoxide (DMSO, a powerful antioxidant and anti-inflammatory),
alpha lipoic acid (ALA or lipoic acid), tocopherols (aka vitamin
E),
glutathione, alpha and beta glucans (immune-boosting, antioxidant and
anti-diabetic),
indoles (remember the anti-cancer antioxidants found in cruciferous
veggies?),
octacosanol, 3-hydroxybutyrate and related hydroxybutyrates
(antioxidants and mitochondrial energy substances), powerful
isothiocyanate antioxidants,
inositol, (likely) enantiomers which may be potent anti-diabetic and
antioxidant
factors, and (likely) quercetin.
Of course, the yeast and lactic acid bacteria in
the brew also produce
a wide range of antioxidants and anti-fatigue (and even anti-diabetic)
agents as well, including glutathione and a zillion others,
and this
production is likely potentized drastically by the hydrogen-rich
reducing
environment offered by the phototrophic organisms.
Summary of Conventional
Antioxidant Properties
We have, so far, all realized that at least some of the benefits are
that it acts as a very beneficial probiotic and also a powerful source
of biochemical antioxidants - that is, complex chemical compounds which
must
be manufactured by life forms (usually vascular plants or
microorganisms.)
Other Antioxidants -- the
Primeval Hydrogen Ion (Hydride species)
Antioxidant
The current author, also a (credentialed) scientist and researcher,
has been able to develop very strong evidence that EM1 also produces,
during
"normal" anaerobic and near-anaerobic fermentation, ample amounts of
the
primitive, primeval hydrogen ion (aka hydride) antioxidant. (note:
the descriptive terms "primitive" and "primeval" are used for this
powerful
antioxidant ion because it was the first antioxidant present on Earth,
and pre-dated the appearance of any life forms on Earth.) This
ion, apparently contained in cages in the water structure, has strong
reducing (antioxidant) properties, and is the same antioxidant hydrogen
(hydride) ion found in so-called "alkaline ionized water" produced by
water
ionizers, which also first became popular in Japan over the past 40
years
before spreading to the rest of the world. The more correct,
descriptive
and accurate term for "alkaline ionized water", by the way, is
"Electrolyzed
Reduced Water", or ERW, and that is the term often used to denote such
a liquid in the peer-reviewed scientific literature (witness the
excellent
articles by Dr. Shirahata over the past 6 years.)
In any case, it appears at this time that, in
addition to all the more
complex biochemical antioxidants produced by EM1, some of which were
listed
above, EM1 fermentation also produces very large amounts of the same
primitive,
primeval antioxidant form of hydrogen found in ERW (the same stuff as
produced
by a water ionizer costing from $650 to $2,500). This finding
does,
of course, make sense for "reducing" bacteria, since related strains of
anaerobic organisms as found naturally occurring in nature have been
reported
(in the scientific literature) to be able, over time, to reduce the
oxidative
"damage" (measured by ORP) to water in deep wells, and instead shift it
to a more reduced (antioxidant) value (meaning a lower ORP.)
One
significant difference between EM1 and a water ionizer, however, is
that
EM1 does not use batteries, wires, electrodes and an electrical power
supply
to produce this primitive hydrogen antioxidant, but rather uses the
complex
relationship between synergistic beneficial microorganisms to create
quantities
of this antioxidant.
If you wish to learn more technical details of
this hydrogen antioxidant
and its presence in EM1 and EM1 products, please proceed to the section
below, entitled The Hydrogen Antioxidant in EM --
More Details
.
The Primeval Hydrogen (Hydride
species) Antioxidant in EM -- More
Details
You may or may not be aware that I have been researching the negative
hydrogen ion as a powerful antioxidant nutrient for years, and that I
have
available a small research laboratory devoted to that and several other
topics. The negative hydrogen ion is found plentifully in
unprocessed
raw plant foods, unprocessed raw animal foods and some "wild" stream
and
well water (unfiltered, unprocessed). For folks wishing to
ingest
it as a supplement, the hydrogen antioxidant has been available for a
number
of years in "alkaline ionized water" from water ionizers, and more
recently,
in Dr. Patrick Flanagan's product called MegaHydrin (also Active
H-).
Why am I telling you all this? Because, in about three
paragraphs,
I will be telling you something quite profound...
As I worked on this update to this webpage EM
antioxidants earlier this
afternoon (early February 2003) at the PC, I had to take some time off
every hour or two to go into my lab and make some detailed measurements
on some new batches of Activated EM (AEM) which I started about 28
hours
earlier. I had made up several batches, and one was very high
in
volcanic/sedimentary rock dust content, as it was what I called my
"monatomic
mineral" batch. Due to my unique culturing and nutrient
methods (most
are described in the section entitled Making AEM/EM
Extension,
my one-gallon AEM jugs were bubbling merrily just 22 hours after I had
started them, and at 26 hours, the pH had already dropped to 4.3, and I
was able to start a series of antioxidant tests for the presence of
hydrogen
ion antioxidants in the AEM brewing in the jugs. Briefly,
when I
had made my previous (at least six) batches of AEM in the past, I had
noticed
something really weird..... evidence that the EM was producing MASSIVE
quantities of the primitive primeval hydrogen antioxidant known as the
hydride ion (same antioxidant found in the so-called "alkaline ionized
water" produced by expensive water ionizers!) This time
around, with
my new batches, I had determined to carefully monitor that possibility
and to control for some variables......
Well, I can now tell you that my AEM brews, at
only 28 hours old, were
not only merrily bubbling (pH now 4.21) along, but that each of the
(cheap
flimsy plastic 1-gallon water jugs) jugs of AEM showed very strong
evidence
that the organisms are producing a truly "reducing" (antioxidant)
environment
even on the atomic level, just as on the bio-chemical level.
For
example, my AEM batch brewed for drinking (a bit of sea salt added, and
one tbsp. of rock dust added) showed an ORP of -338 mv, which is
amazing!
Stranger, my "monatomic" batch, which was similar to the nutritional
batch
but had 6 tbsp. of rock dust (Azomite, plus a bit of Pascalite) added
as
well, showed an ORP of -440 mv.
What is weirder yet is that you must appreciate
that these ORP measurements
were made at a rather acid pH of 4.35. As described by
Nernst's law,
ORP readings are interdependent upon pH, and thus, a fixed amount of
hydrogen
antioxidant (aka "reducing power") which would produce an ORP of -300
mv
at a pH of 9.2 would read an ORP of only about +150 at a pH of 4.2,
although
the antioxidant ("reducing") power would remain the same.
Thus, it
must be appreciated that for an ORP meter to show an ORP in the range
of
-330 or -430 mv at a pH of 4.4, the "reducing" (antioxidant) power of
the
AEM brew must be ENORMOUS.
If you feel that you really must learn a bit more
about the whole class
of primitive primeval hydrogen ion-based (aka hydride) antioxidants,
you
may wish to take a look at the off-site link which follows: the author
of this site also operates a website devoted to the primitive hydrogen
antioxidants, called the
H-minus
Ion Website, at http://www.h-minus-ion.org .
Even though we have technically known that the EM
organisms are technically
"reducing" rather than oxidizing, this is strong evidence that the AEM
brews contain even ample levels of the most primitive and primeval and
ancient antioxidant in the world -- at the atomic level, and one which
predates the existence of all life forms on Earth -- the
hydrogen
(hydride) antioxidant. More fascinating yet, in 24 hours of
brewing,
my AEM jugs have produced reduced water (well, antioxidant liquid) with
a reducing measurement better than any counter-top water ionizer other
than a $2,400 batch device.
Update -- Further Observations on
Reducing Activity and ORP in AEM
Brews
I have found that the ORP of batches such as described above (brewed
with freshly Activated EM, and at relatively high temperatures, such as
94 to 100 degrees F) often usually drops again within 24 hours of
peaking
(usually at about -500 mv), first to the -100 mv. range, then to about
zero, and then will slowly, over the next two months, rise to about
+140
mv (less if stored totally anaerobically; this ORP reading may vary
from
batch to batch dependent upon a number of variables.) On the
other
hand, I have made several batches of AEM (using less active starter
cultures
and lower brewing temperatures in the 87 degree F range) where the ORP
fell to the -400 range within 60 hours of starting, and stayed below
-165
for another 7 days before slowly starting to rise again. The
variables
affecting how long the ORP will remain in a strongly reducing (low ORP)
area seem to be:
- degree to which an anoxygenic (anaerobic) is
maintained, versus some degree
of aerobic brewing
- activity and vigor of starter culture
- brewing temperature
- whether sea salt was added, as well as quantity
- whether rock dusts (minerals) were added, as
well as quantity
- type and quantity of molasses used
As the brew ages, and the ORP slowly rises, what happens to the ORP and
the reducing activity? Are they truly dissipating away as ORP
regresses
toward zero or positive realms? Of course, it is true that
the ORP
will peak whenever the phototropic organisms are experiencing a feeding
and growth spurt, and producing hydrogen and hydrogen ions.
It is
also true that some of the hydrogen causing this ORP shift will
eventually
escape, as H2 gas (common diatomic hydrogen), by dissipating through
the
walls of the container. However, at this time, it appears that the
appearance
of the strongly reducing environment (with ORPs to -500 or
below)
is actually providing raw materials to allow the microorganisms to
build
more complex bio-chemical antioxidants (ubiquinone, quinones,
polyphenols,
inositol, etc.) within the brew, many of which store the H-minus ion in
their antioxidant structure (to be donated at the proper time), so
nothing
is lost!
I have been able to observe that the recession of
ORP after the strong
reducing peak will be all the more accelerated if you are allowing some
aerobic activity (via air/oxygen entering the brew) by
opening the
container daily and taking ORP or pH measurements. Indeed, a way to
maximize
the strongly negative ORP is to brew the AEM in the following manner:
- brew in thick glass-wall containers with some
type of pressure release valve
in the cap which will vent at about 2 to 4 psi, well before the bottle
might explode from internal pressure
- keep contents of bottle as anaerobic as
possible -- exclude air and oxygen
- allow to ferment for at least a week (at about
90 F) before opening
- but, lastly, do not panic if the ORP
does drop from its peak; as related
above, it appears that this reducing environment is actually providing
raw materials to allow the microorganisms to build more complex
bio-chemical
antioxidants (ubiquinone, quinones, polyphenols, inositol, etc.) within
the brew, so nothing is lost!
Developments
My friend and fellow researcher Dr. Patrick Flanagan, inventor of
Megahydrin
(aka Active H-) and the Neurophone, offered in early 2003 to have a
series
of NMR (Nuclear Magnetic Resonance) spectroscopic analyses performed on
some samples of the AEM to help determine the exact signature and the
"energy
level" of the hydride (negative hydrogen ions) present in the
liquid.
According to a report I received from Dr. Flanagan in March 2003, the
results
of these proprietary tests, some of which were performed on samples of
EM brews I have made for human use, confirmed the presence in the EM
brews
of extremely large amounts of a number of the primitive hydride
species,
as well as higher-order more complex biochemical antioxidants, many
of which also contain the hydride ion and which donate it in order to
act
as an antioxidant in vivo (in the body).
Accuracy, Care and Cleaning of ORP and pH
Meter Electrodes
Those working with EM may often wish to use digital pH or ORP meters
to assess the progress, stage or performance of their EM or AEM
brew.
Such digital meters employ electrodes (made of glass, plastic, metal
and
a reference liquid) for measurement, which are immersed in each sample
to be tested. Even under the very best of circumstances, ORP
electrodes
will experience some degradation of readings, often severe. Usually the
first thing to suffer is measures of strongly negative ORP -- such
readings
will regress strongly toward the +200 range -- due to deposits on the
electrode
or even ion migration into the electrode insulator (more of a problem
with
plastic electrodes.)
To a somewhat lesser extent, the same is true of pH electrodes, and
they will experience some degradation unless cleaned
regularly. Since
pH is a logarithmic measure, the effect of dirty electrodes on pH
readings
will usually be far less severe than with ORP electrodes.
The whole area of cleaning ORP and pH electrodes is more of an art
than a science. I prefer not to delve deeply into
the topic
here, beyond saying that soaking an electrode in a mixture of 1 part
chlorine
bleach to 3 or 4 parts water (careful! such a mixture is hazardous to
your
skin and eyes!) for 8 minutes, followed by vigorous rinsing in cold or
lukewarm water, followed by soaking for 20 minutes in a good volume of
distilled water, will often help matters a lot.
For further information on care of ORP and pH electrodes, please see
any of all of the following off-site webpages, which tell the story far
better than I could:
http://www.eutechinst.com/techtips/tech-tips19.htm
http://www.hannacan.com/techsupport/advice_orp.htm
http://www.automatedaquariums.com/tecclean.htm
From Which Organism Does the Hydrogen
Antioxidant Come?
As for the primitive primeval hydrogen antioxidant, I can venture a
strong guess that it is primarily produced by the phototrophic
organisms;
such organism are famous in the scientific literature for acting as
strong
reducing agents, and indeed, a number of researchers are currently
working
on using such organism to produce hydrogen gas from wastes.
However,
as Dr. Higa has consistently reminded staffers and farmers, there is a
tremendous synergistic interdependence between the organisms.
Even
though we could likely prove that it is the phototrophic organisms in
EM1
which are actually producing the hydrogen antioxidant, it is
inescapably
true this production of this physical antioxidant is dependent upon a
complex,
interdependent and synergistic relationship between the three major
groups
of organisms, and likely the helper organisms as well.
EM-X Antioxidant Supplement
Liquid
The EMCO organization markets
an antioxidant liquid supplement (microbe-free; via micro-filtering),
made
from a bran/kelp EM fermentation, which is claimed to be very high in
important
nutrients and antioxidants. This juice, named EM-X, is quite expensive,
and yet some folks have reported some good results from it.
From
what I can decipher from what is disclosed by the EM folks on the web,
it appears that they manufacture EM-X by first making batches of rice
bran,
sea vegetables and other nutrients, and fermenting it anaerobically
with
EM for up to 3 months, followed by one more fermentation stage, and
then
by micro-filtering with a 0.22 micron filter (and some assert that it
is
sterilized by pasteurization as well.) They reveal that they
micro-filter
the liquid and otherwise "process it" to remove all
microorganisms.
This probably also drastically increases shelf life, plus, of course,
there
are then no "germs" left to scare off germ-phobic consumers who might
not
know any better.
EM-X (pasteurized
filtered version) box and bottle
as marketed in Japan
Dr. Higa and the EMCO folks
claim that the EM-X brew contains a whole wide range of antioxidants as
well as a host of other beneficial substances from the rice bran, and
indeed,
that is the whole selling point of the EM-X liquid.
I personally find EM-X to
be way too expensive to use (and in any case, I have no significant
health
problems, and I eat a wonderful largely-raw and largely-Paleolithic
diet)
as it sells in the USA for about $70 per pint, and the recommended
dosage
is a minimum of one teaspoon per day. Several EM enthusiasts
whom
I have met in the beyond-organic and uber-organic farming communities
tend
to view EM-X with scorn, saying that it is largely "dead", due to the
micro-filtering
and other forms of processing. I tend to agree; while I am
sure that
EM-X does offer wonderful nutrients and benefits, I would much rather
make
my own "live" EM products here. On the other hand, I must
note that
I have heard some wonderfully positive stories from many folks who have
tried using commercially-available EM-X with good success.
EM-X2
I have learned as of February
2003 that there is now available, at least in Japan, a slightly
lower-quality
EM-X product named EM-X2, which sells for about 15% cheaper than the
(very
high-priced) EM-X.
For Techies and Scientists
Only: Antioxidant Activity of EM versus ORP Measures
It appears to be true that
the organisms in EM perform their digestion of substances largely via
"reducing"
(literally "antioxidant") activities rather than "oxidizing"
activities,
and it is also true that the EM organisms produce a wide range of
antioxidants
as they work and grow. If the brew is fermented in even a fairly
anaerobic
manner, you will always be able to observe (if you watch closely
enough,
with a good meter....) the ORP peak in the reducing direction to at
least
-340 to -550 mv (this excursion into a moderately strong reducing
region
will last from anywhere from 2 or 3 days to 8 days, depending upon a
number
of factors about the brew), before again slowly regressing toward zero
or the low positive range.
Please, however, bear in
mind that this reducing (antioxidant) activity and the antioxidants
themselves
may or may not show up as a persistent and consistently lower ORP (or
negative
ORP) of the Activated EM (aka EM Extension) liquid or other EM liquid
products;
it is often only primitive primeval hydrogen-ion-based antioxidants
(aka
primeval hydride antioxidants, such as "alkaline" ionized reduced water
[aka ERW], or solid hydrogen/hydride supplemental products such as
MegaHydrin, for the most part) which tend to drastically lower ORP of
water and aqueous
solutions, and even those forays decay over time (usually over a few
days
to two weeks.) In EM and AEM, the evidence is that the organisms in the
brew are capturing and using much of the reducing power (as evidenced
by
the low ORP) in the liquid to use as fuel with which to build even more
complex antioxidants (biochemical antioxidants...) and other
nutrients.
The more sophisticated biochemical antioxidants (e.g., vitamin E,
COQ10,
quinones, ALA, etc.) made by living organisms tend to hold onto their
H-ions
too tightly (in complex biochemical molecules) to influence ORP much at
all, so they will not influence ORP readings in a strongly reducing
(negative)
direction.
Once again, however, even
the temporary decreases (peaking usually between -340 and -550 mv) of
ORP
seen in the AEM (aka EM Extension) as it brews, are all the more
amazing
because the pH is constantly plummeting, and the final pH is very low,
about 3.6. Nernst's Laws and Equations state that for a given amount of
primitive hydrogen antioxidants available, the ORP will, essentially,
read
far higher at lower pHs than at high pHs. In other words, ORP
is
a RELATIVE scale, interdependent with pH.... So, for example, a
solution
with an ORP of +200 at a pH of 3.6 can actually indicate as much (or
more)
reducing power as a solution with an ORP of -250 at a pH of 11.0.
As noted earlier, if
you feel that you really must learn a bit more about the whole class of
primitive primeval hydrogen ion-based (aka hydride) antioxidants, you
may
wish to take a look at the off-site link which follows: the author of
this
site also operates a website devoted to the primitive hydrogen
antioxidants,
called the
H-minus Ion Website,
at http://www.h-minus-ion.org .
For Radical Experimenters
-- Making an EM-X-like Beverage
See the end of the section
entitled Human
Uses -- EM Products for Drinking, Bathing, Brewing an EM-X-like
Antioxidant-rich
and Nutrient-rich Probiotic Beverage
(found on a later
page in this series) for a few quick hints on how to make EM-X at
home.
This sub-section will eventually be expanded into a complete section by
itself.
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